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Rizka Amalia | B1K014038

Seminar Proposal
Presenter Rizka Amalia | B1K014038
Waktu Mulai 31 January 2018 - 10:00am
Waktu Selesai 31 January 2018 - 11:00am
Lokasi Ruang Rapat Ex-Bioling
Judul Seminar

The Survival and Morphological Character of Primary and Secondary Kidney Cell Culture in Media Containing Different Type of Sera

Pembimbing I Dra. Gratiana EW, M.Rep.Sc., Ph.D.
Pembimbing II Atang, S.Si., M.Si.
Pembimbing III
Penelaah I Endah Sri Palupi, S.Si., M.Sc.
Penelaah II Dr. Sorta Basar Ida S, M.Si.
Penelaah III
Sinopsis

The fish kidney of hard-lipped barb (Osteochilus vittatus) as a source of cell culture is still limited. In culturing such cells, the supplementation of culture media with the combination between fetal bovine serum (FBS) and fish serum (FS) will be used to determine best suitable media containing 100% FBS, 50% FBS:50% FS, and 100% FS. Those will be subjected to the experiment using Completely Randomized Design (CRD). The cell survival will be evaluated based on parameters: cell viability, cell density, PDL, cell morphology, and cell proportion. Nine replications will be conducted for each treatment. The data will be analyzed using one-way ANOVA and Tukey’s Post-Hoc test.
Keywords: kidney, fish serum, FBS, hard-lipped barb

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