Fakultas Biologi

Change Font Size

Change Screen

Change Layouts

Change Menu Styles

Cpanel

CITRA INNEKE WIBOWO | B1J012010

Pendadaran
Kandidat Sarjana CITRA INNEKE WIBOWO | B1J012010
Waktu Mulai 11 April 2017 - 9:00am
Waktu Selesai 11 April 2017 - 10:30am
Lokasi R. Rapat Ex. Zoologi
Penelaah I Drs. Eddy Tri Sucianto, MP.
Penelaah II Dra. Kamsinah, M.P.
Penelaah III
Penelaah IV
Penelaah V
Judul Skripsi

Efektivitas Bacillus Thuringiensis Dalam Pengendalian Larva Nyamuk Anopheles Sp.

Sinopsis Skripsi

Nyamuk Anopheles sp adalah vektorpenyakit malaria. Malaria merupakan salah satu penyakit infeksi parasit yang disebabkan oleh Plasmodium dan ditularkan oleh nyamuk betina Anopheles sp. Penyebaran Anopheles sp. di Indonesia bersifat lokal spesifik artinya spesies Anopheles sp. yang ditemukan di suatu wilayah dipengaruhi oleh kespesifikan habitat perkembangbiakannya. Pengendalian vektor penyakit malaria dapat dilakukan secara biologis yaitu dengan menggunakan Bacillus thuringiensis. Mekanisme pengendalian parasite tersebut adalah dengan cara memproduksi toksin yang dapat mematikan larva nyamuk.

Tujuan penelitian adalah untuk mengetahui efektivitaskonsentrasi Bacillus thuringiensis dalam pengendalian larva nyamuk Anopheles sp., mengetahui pengaruh stadia larva Anopheles sp.terhadap efektivitas B. thuringiensis dalam pengendalian larva nyamuk Anopheles sp., mengetahui pengaruhinteraksi antara konsentrasi B. thuringiensis dan stadia larva dalam pengendalianlarva nyamuk Anopheles sp.Penelitian ini dilakukan secara eksperimental menggunakan Rancangan Acak Lengkap Faktorial (RAL Faktorial) yang terdiriatasdua faktor yaitu konsentrasi Bacillus thuringiensis dan stadia larva Anophelesdengan pengulangan tiga kali. Perlakuan yang dicobakan adalahkonsentrasi Bacillus thuringiensis (A) yang terdiri atas 5 taraf:A0: konsentrasi B.thuringiensis0 CFU.mL-1, A1: konsentrasi B.thuringiensis102 CFU.mL-1, A2: konsentrasi B.thuringiensis104 CFU.mL-1, A3: konsentrasi B.thuringiensis106CFU.mL-1, A4: konsentrasi B.thuringiensis108 CFU.mL-1. Perlakuan tahapan instar larva Anopheles sp. (B) adalah sebagai berikut:B1: stadia larva instar I, B2: stadia larva instar II, B3: stadia larva instar III, B4: stadia larva instar IVsehingga terdapat 60satuan percobaan.Parameter yang diamatiadalah kematian larva Anopheles tiap satuan percobaan dan populasiB. thuringiensispada larva.

Hasil penelitian  menunjukkan konsentrasi B. thuringiensis isolat CK dan IPB CC yang paling berpengaruh dalam pengendalian larva Anopheles sp adalah 108 CFU.mL-1 . Instar larva yang paling peka terhadap B. thuringiensis isolat IPB CC adalah instar I dan II sedangkan instar yang peka terhadap isolat CK adalah instar II, Perlakuan konsentrasi isolat B. thuringiensis dan tingkat instar larva yang paling baik dalam pengendalian larva Anopheles sp. adalah 108 CFU.mL-1, dan instar I dan II.

 

Anopheles mosquito is a vector of malaria. Malaria is a parasitic infection caused by Plasmodium and transmitted by the female Anopheles mosquito. The spread of Anopheles sp. in Indonesia is local Anopheles species specific meaning were found in a region affected by breeding habitat specificity. Malaria vector control can be done biologically by using Bacillus thuringiensis mekanism of controlling that parasite is works by producing a toxin that can kill larvae of mosquito.

The purpose of the study was to determine the effectiveness of the objective of the research was to determine the effectiveness of Bacillus thuringiensis concentration in the control of larvae of Anopheles sp., to know the influence larval stage Anopheles sp. the effectiveness of B. thuringiensis to control of Anopheles sp. larvae, determine the effect of interaction between the concentration of B. thuringiensis and larval stage in the control of Anopheles sp. larvae the concentration of Bacillus thuringiensis in the control of Anopheles sp. larvae, knowing the influence larval stage Anopheles sp. the effectiveness of B.thuringiensis in the control of Anopheles sp. larvae, determine the effect of interaction between the concentration of B. thuringiensis and larval stage in the control of Anopheles sp. larvae. This research was carried out experimentally using factorial completely randomized design (RAL Factorial) consisting of two factors: the concentration of bacteria Bacillus thuringiensis and Anopheles larval stage with three replications. The treatment is carried out for 3 weeks at a concentration of Bacillus thuringiensis (A) will be done in this study are as follows: A0: concentration of B.thuringiensis 0 CFU.mL-1, A1: the concentration of B.thuringiensis 102 CFU.mL-1, A2: the concentration of B.thuringiensis 104 CFU.mL-1, A3: concentration of B.thuringiensis 106 CFU.mL-1, A4: the concentration of B.thuringiensis 108 CFU.mL-1.1st instar larval stages while treatment Anopheles sp. (B) are as follows: B1: the first instar larval stage, B2: second instar larval stage, B3: the third instar larval stage, B4: IV instar larval stage so that there are 60 experimental unit. Parameters measured were death Anopheles mosquitoes each experimental unit and the number of existing B.thuringiensis on larvae.

The results showed the concentration of B. thuringiensis isolates CK and IPB CC most influential in controlling larvae of Anopheles sp is 108 CFU.mL-1. Instar larvae are most sensitive to B. thuringiensis isolates IPB CC is instar I and II while instar sensitive bacterial isolation results (CK) is the second instar, treatment concentration of isolates of B. thuringiensis and level instar larvae of most good in controlling the larvae of Anopheles sp , is 108 CFU.mL-1, and instar I and II.

Halaman ini »