Fakultas Biologi

Change Font Size

Change Screen

Change Layouts

Change Menu Styles

Cpanel

Embryogenic Callus Induction From Male Inflorescence of Local Banana Cultivars With a View to Produce Fusarium Wilt Resistant Plant Via In Vitro Selection

JudulEmbryogenic Callus Induction From Male Inflorescence of Local Banana Cultivars With a View to Produce Fusarium Wilt Resistant Plant Via In Vitro Selection
Publication TypeConference Proceedings
Year of Publication2010
AuthorsSugiyono, Yuniaty A, Prayoga L
Conference Name2nd International Conference on Biosciences and Biotechnology " Pave The Way to A Better Life"
PaginationOA42-OA47
Date Published09/2010
PublisherUniversitas Udayana
Conference LocationBali
ISBN978-602-9042-11-5
ISBN Number978-602-9042-11-5
Keywords24-D, Banana, embryogenic callus, IAA
Abstract

This I-MHERE funded research is the first step of a research program to produce Fusarium wilt resistant of local banana cultivars using in vitro selection approach. The objecitves of this first research step are to produce sufficient material for in vitro selection. The specific objectives of this experiment was to study the influence of auxin (2,4-D and IAA) on embryogenic callus formation from male inflorescence of four local banana cultivars (Raja, Ambon, Ambon Nangka, Kapok Kuning). An experimental research method on a split-split plot design has ben used. The main plot was local banana cultivars (Raja, Ambon, Ambon Nangka, Kapok Kuning), the sub plot was the kind of auxin (2,4-D, IAA), and the sub-sub plot was auxin concentration (0; 5; 10; 15 µM). All treatment combinations were replicated 3 times. The nutrient medium used was Murashige and Skooge (MS-1962) supplemented with 7.5 µM BAPand solidified with 0.8 % agar.The cultures were kept in dark condition at 24 ⁰C for six weeks. The paramaters measured include the precentage of callus formation, callus formation time, and the type of callus formed. The research results showed that male inflorescence can be used as explants for mass production of embryogenic calli of 4 local banana cultivars (Raja, Ambon, Ambon Nangka, Kapok Kuning). “Raja” cultivar was found to be the most responsive cultivar leading to the highest percentage of callus formation and short callus formation time. In addition, it was also found that no significance difference between 2,4-D and IAA on both callus formation time and the percentage explants which produced callus, however 2,4-D resulterd in better embryogenic calli formation than IAA. Callus formation was controlled by the concentration of auxin applied. The higher the concentration applied, the higher the precentage of explants which produced callus.

SisipanUkuran
Embryogenic Callus Induction From Male Inflorescence of Local Banana Cultivars With a View to Produce Fusarium Wilt Resistant Plant Via In Vitro Selection.pdf1.08 MB