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Uji Perbedaan Media dan Kkonsentrasi BAP Terhadap Pertumbuhan Tunas Pisang Raja Secara Kultur In Vitro

JudulUji Perbedaan Media dan Kkonsentrasi BAP Terhadap Pertumbuhan Tunas Pisang Raja Secara Kultur In Vitro
Publication TypeJournal Article
Year of Publication2010
AuthorsPrayoga L, Sugiyono
JournalJurnal Ilmu-Ilmu Pertanian Universitas Muhammadiyah Purwokerto
VolumeXII
Pagination89 - 99
Date Published12/2010
ISSN1411-1063
Abstract

A research with aims of obtaining which better between MS and Gamborg (B5) as the media in raja banana in vitro culture and knowing how much BAP needed in order to gain a most optimal growth of raja banana in in vitro culture. The research has been done in Plant Physiology Laboratory Biology Faculity General Soedirman University from April up to July 2010. Completely Randomized Design has been used with split plot design. As the main plot were media; i.e. MS media (M1) and Gamborg (B5) media (M2). As the sub plot were BAP concentration (K); consist of  K0: 0 µM, K1: 5 µM, K2: 10 µM, K3:15 µM, K4: 20 µM and K5: 25 µM.  Each treatment repeated three times and each repeating treatments consist of three sub-samples. It could be concluded from the result that raja banana in vitro culture might be done in MS or Gamborg(B5) media. The best concentration of  BAP in stimulating the foming and the growth of raja banana shoots in in vitto culture was  15 µM. Key word : BAP, Gamborg (B5) mediun, MS medium, Raja banana shoot.