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Isolation and characterization of 14 microsatellite markers for Rhizophora mucronata (Rhizophoraceae) and their potential use in range-wide population studies

JudulIsolation and characterization of 14 microsatellite markers for Rhizophora mucronata (Rhizophoraceae) and their potential use in range-wide population studies
Publication TypeJournal
Year of Publication2012
AuthorsShinmura Y, Wee AKS, Takayama K, Meenakshisundaram SH, Asakawa T, Onrizal, Adjie B, Ardli ER, Sungkaew S, Malekal NB, Salmo SG, Yllano OB, Saleh NM, Tung NX, Soe KK, Oguri E, Murakami N, Watano Y, Baba S, Webb EL, Kajita T
Keywordsgenetic diversity, mangrove, Pyrosequencing, SSR
Abstract

A set of 14 new microsatellite markers was developed for mangrove species Rhizophora mucronata (Rhizophoraceae) by using pyrosequencing. Fifty-six samples from 9 populations of R. mucronata in the Indo-West Pacific region were genotyped; all loci were polymorphic, with the number of alleles ranging from 2 to 9. The mean expected heterozygosity per locus was 0.16 in a population from Sabah, no significant linkage disequilibrium was found among loci, and significant deviation from Hardy–Weinberg equilibrium was found in 3 loci. The polymorphic microsatellite markers with samples covering most of the species’ distribution range can be applied in genetic diversity studies covering a broad geographical range of the species.

DOI10.1007/s12686-012-9681-y